Blocking Assay:Article Title: Tenascin C + myofibroblasts exacerbate vascular neointimal hyperplasia by propagation of nerve-macrophage interactions in mice.
Article Snippet: After incubating Mu/Rb-HRP secondary antibody (AiFang biological, AFIHC001) for 1 h at RT, developed by staining with DAB kit (Servicebio, G1212) for 1min. .. The sections were blocked with blocking buffer (5% BSA and 0.2% Triton X-100 in PBS) for 1 h and stained with anti-TNC (1:200, Proteintech, 67710-1-Ig), anti-Ki67 (1:200, Servicebio, GB121141), anti-αSMA (1:500, Cell Signaling Technology, 48938S), anti-Fn1 (1:200, Proteintech, 66042-1-Ig), anti-Postn (1:200, Proteintech, 66491-1-Ig), antiCD68 (1:200, BIO-RAD, MCA1957), anti-CD11c (1:200, Cell Signaling Technology, 97585), anti-F4/80 (1:200, Proteintech, 29414-1-AP), antiPostn (1:200, Proteintech, 66491-1-Ig), anti-Synapsin1/2 (1:100, SYSY, 106 002), anti-TNF (1:200, Proteintech, 17590-1-AP), anti-NOS2 (1:200, Invitrogen, PA1-036), anti-ARG1(1:200, Cell Signaling Technology, 93668), anti-CCL2(1:200, Proteintech, 26161-1-AP) anti-CD45 (1:200, Abcam, ab10558) and anti-βIII-tubulin (1:1000, Abcam, ab78078) primary antibodies overnight at 4 °C. .. Afterwards, the sectionswere rinsed three times in PBS and stained with corresponding secondary antibodies (all from Invitrogen) for 1 h at RT.
Article Title: Tenascin C + myofibroblasts exacerbate vascular neointimal hyperplasia by propagation of nerve-macrophage interactions in mice
Article Snippet: After incubating Mu/Rb-HRP secondary antibody (AiFang biological, AFIHC001) for 1 h at RT, developed by staining with DAB kit (Servicebio, G1212) for 1 min. .. The sections were blocked with blocking buffer (5% BSA and 0.2% Triton X-100 in PBS) for 1 h and stained with anti-TNC (1:200, Proteintech, 67710-1-Ig), anti-Ki67 (1:200, Servicebio, GB121141 ), anti-α-SMA (1:500, Cell Signaling Technology, 48938S), anti-Fn1 (1:200, Proteintech, 66042-1-Ig), anti-Postn (1:200, Proteintech, 66491-1-Ig), anti-CD68 (1:200, BIO-RAD, MCA1957), anti-CD11c (1:200, Cell Signaling Technology, 97585), anti-F4/80 (1:200, Proteintech, 29414-1-AP), anti-Postn (1:200, Proteintech, 66491-1-Ig), anti-Synapsin1/2 (1:100, SYSY, 106 002), anti-TNF (1:200, Proteintech, 17590-1-AP), anti-NOS2 (1:200, Invitrogen, PA1-036), anti-ARG1(1:200, Cell Signaling Technology, 93668), anti-CCL2(1:200, Proteintech, 26161-1-AP) anti-CD45 (1:200, Abcam, ab10558) and anti-βIII-tubulin (1:1000, Abcam, ab78078) primary antibodies overnight at 4 °C. .. Afterwards, the sections were rinsed three times in PBS and stained with corresponding secondary antibodies (all from Invitrogen) for 1 h at RT.
Staining:Article Title: Tenascin C + myofibroblasts exacerbate vascular neointimal hyperplasia by propagation of nerve-macrophage interactions in mice.
Article Snippet: After incubating Mu/Rb-HRP secondary antibody (AiFang biological, AFIHC001) for 1 h at RT, developed by staining with DAB kit (Servicebio, G1212) for 1min. .. The sections were blocked with blocking buffer (5% BSA and 0.2% Triton X-100 in PBS) for 1 h and stained with anti-TNC (1:200, Proteintech, 67710-1-Ig), anti-Ki67 (1:200, Servicebio, GB121141), anti-αSMA (1:500, Cell Signaling Technology, 48938S), anti-Fn1 (1:200, Proteintech, 66042-1-Ig), anti-Postn (1:200, Proteintech, 66491-1-Ig), antiCD68 (1:200, BIO-RAD, MCA1957), anti-CD11c (1:200, Cell Signaling Technology, 97585), anti-F4/80 (1:200, Proteintech, 29414-1-AP), antiPostn (1:200, Proteintech, 66491-1-Ig), anti-Synapsin1/2 (1:100, SYSY, 106 002), anti-TNF (1:200, Proteintech, 17590-1-AP), anti-NOS2 (1:200, Invitrogen, PA1-036), anti-ARG1(1:200, Cell Signaling Technology, 93668), anti-CCL2(1:200, Proteintech, 26161-1-AP) anti-CD45 (1:200, Abcam, ab10558) and anti-βIII-tubulin (1:1000, Abcam, ab78078) primary antibodies overnight at 4 °C. .. Afterwards, the sectionswere rinsed three times in PBS and stained with corresponding secondary antibodies (all from Invitrogen) for 1 h at RT.
Article Title: Tenascin C + myofibroblasts exacerbate vascular neointimal hyperplasia by propagation of nerve-macrophage interactions in mice
Article Snippet: After incubating Mu/Rb-HRP secondary antibody (AiFang biological, AFIHC001) for 1 h at RT, developed by staining with DAB kit (Servicebio, G1212) for 1 min. .. The sections were blocked with blocking buffer (5% BSA and 0.2% Triton X-100 in PBS) for 1 h and stained with anti-TNC (1:200, Proteintech, 67710-1-Ig), anti-Ki67 (1:200, Servicebio, GB121141 ), anti-α-SMA (1:500, Cell Signaling Technology, 48938S), anti-Fn1 (1:200, Proteintech, 66042-1-Ig), anti-Postn (1:200, Proteintech, 66491-1-Ig), anti-CD68 (1:200, BIO-RAD, MCA1957), anti-CD11c (1:200, Cell Signaling Technology, 97585), anti-F4/80 (1:200, Proteintech, 29414-1-AP), anti-Postn (1:200, Proteintech, 66491-1-Ig), anti-Synapsin1/2 (1:100, SYSY, 106 002), anti-TNF (1:200, Proteintech, 17590-1-AP), anti-NOS2 (1:200, Invitrogen, PA1-036), anti-ARG1(1:200, Cell Signaling Technology, 93668), anti-CCL2(1:200, Proteintech, 26161-1-AP) anti-CD45 (1:200, Abcam, ab10558) and anti-βIII-tubulin (1:1000, Abcam, ab78078) primary antibodies overnight at 4 °C. .. Afterwards, the sections were rinsed three times in PBS and stained with corresponding secondary antibodies (all from Invitrogen) for 1 h at RT.
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